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ÀÛ¼ºÀÏ : 16-09-09 08:58
Rotifer Culture Protocol 2
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±Û¾´ÀÌ :
¿î¿µÀÚ
Á¶È¸ : 9,753
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< Rotifer Culture Protocol 2>
¡Ø À¯ÀÇ»çÇ× ¨ç ¹è¾çÇÒ ¸ðÁ¾ÀÇ rotifer ¼ö°¡ ÀûÀ» °æ¿ì, ȸ¼öÇÏ´Â rotifer ¼öµµ Àû¾îÁø´Ù. ¨è °°Àº ¿ë·®ÀÇ CH(chlorella)¸¦ ±¸¸ÅÇÒÁö¶óµµ, CHÀÇ ³óµµ´Â ´Ù¸¦ ¼ö ÀÖÀ¸¹Ç·Î À¯ÀÇÇÑ´Ù.
1. Material - »ì¾ÆÀÖ´Â rotifer (¸ðÁ¾) - Rotifer food·Î »ç¿ëµÉ CH (´ë»ó´ã¼ö³óÃà Chlorella) - Rotifer »çÀ°À» À§ÇÑ 1L, 2L Çöó½ºÆ½ º´ - È÷ÅÍ(28µµ À¯Áö) - Æø±â(tubing, air stone, air pump) - Rotifer ¼ö°ÅÇÒ ¶§, »ç¿ëÇÒ mesh(#350) - culture solution: KW10(10ppt sea salt/RO)
2. Condition - ¼ö¿Â 28µµ À¯Áö, Æø±â
3. Time table ¨ç 00 dpi [¾Æħ] ¥¡) inoculation & feeding A ¢¸ C(¸ðÁ¾) 400mL + KW10 1200mL ¢º feeding CH 1.0mL B ¢¸ C(¸ðÁ¾) 200mL + KW10 600mL ¢º feeding CH 0.5mL C ¢¸ C(¸ðÁ¾) 200mL + KW10 600mL ¢º feeding CH 0.5mL ¡Ú C(¸ðÁ¾) ¡Ö 1,500ea/mL ¥¢) 6mm tubing¿¡ air stone 1°³¸¦ ³¢¿î ÈÄ, Çöó½ºÆ½ º´¿¡ ³Ö´Â´Ù. ¥£) Æø±â(aeration)
[Àú³á] feeding A : green color ¢º feeding CH 0.8mL (´ë·«) B : green color ¢º feeding CH 0.3mL (´ë·«) C : green color ¢º feeding CH 0.3mL (´ë·«) ¡Ú ½ÇÇè½Ç ³» rotifer »ç¿ë·®¿¡ µû¶ó, ¹è¾ç¿ë±âÀÇ ¿ë·®À» ´Ã·Áµµ µÇÁö¸¸, C(¸ðÁ¾, ³ªÁß¿¡ ¹è¾çÇÒ rotifer)ÀÇ ¿ë·®ÀÌ Å¬¼ö·Ï ¼öÁúÀÌ »¡¸® ¾ÇȵǹǷÎ, C(¸ðÁ¾)´Â Å« ¿ë±â¸¦ »ç¿ëÇÏÁö ¾Êµµ·Ï ÇÑ´Ù. ¡Ú ¨è 01 dpi [¾Æħ] Photos & feeding A : green + brown color(³ì°¥»ö) ¢º feeding CH 1.0mL B : green + brown color(³ì°¥»ö) ¢º feeding CH 0.5mL C : green + brown color(³ì°¥»ö) ¢º feeding CH 0.5mL [Àú³á] feeding A : green > brown color ¢º feeding CH 1.0mL B : green > brown color ¢º feeding CH 0.5mL C : green > brown color ¢º feeding CH 0.5mL
¨é 02 dpi [¾Æħ] Photos & feeding A : brown color ¢º feeding CH 1.0mL B : brown color ¢º feeding CH 0.5mL C : brown color ¢º feeding CH 0.5mL [Àú³á] feeding A : green > brown color ¢º feeding CH 1.0mL B : green > brown color ¢º feeding CH 0.5mL C : green > brown color ¢º feeding CH 0.5mL
¨ê 03 dpi [¾Æħ] Photos & recovery A : brown color ¢º recovery B : brown color ¢º recovery C : brown color ¢º inoculate to A¡®, B¡¯, C¡®
4. Recovery ¹æ¹ý [¾Æħ¿¡ recovery] ¹è¾çÇÑÁö 3ÀÏ° µÈ rotifer recovery A, B : #350(Fig. B)¸¦ »ç¿ëÇÏ¿© recovery ÈÄ, zebrafish¿¡°Ô ±Þ¿©. recovery ÇÒ ¶§, ħÀü¹°ÀÌ ¸¹ÀÌ µé¾î°¡Áö ¾Êµµ·Ï ÇÑ´Ù. recovery ÇÑ rotifer´Â ¹Ýµå½Ã ÇÏ·ç¿¡ ¸ðµÎ ¼Òºñ. C : inoculate to A¡®, B¡¯, C¡®
¡Ø À¯ÀÇ»çÇ× ¨ç rotifer¸¦ ±Þ¿©ÇÑ fry´Â ¹Ýµå½Ã (1ppt sea salt/RO)¿¡¼ »çÀ°ÇÑ´Ù.
¡Ø Tip ¨ç ¾Æħ¿¡ CH¸¦ ±Þ¿©ÇÑ ÈÄ, Àú³á¿¡ brown color·Î º¯ÈÇÏ´Â Áö È®ÀÎÇÑ´Ù. brown color´Â rotifer°¡ Àß Áõ½ÄÇÏ°í ÀÖ´Ù´Â ÀǹÌÀÓ°ú µ¿½Ã¿¡ CH°¡ ¸¹ÀÌ ¼ÒºñµÇ¾ú´Ù´Â ÀǹÌÀÌ´Ù. colorÀÇ º¯ÈÁ¤µµ¸¦ È®ÀÎÇϸç ÀÏÀÏ ±Þ¿©·®À» °áÁ¤ÇÑ´Ù. ¨è ¾Æħ¿¡ CH¸¦ ±Þ¿©ÇÑ ÈÄ, Àú³áÀÌ µÇ¾ú´Â µ¥µµ, color º¯È°¡ ¾ø´Ù¸é ¹è¾ç¿¡ ¹®Á¦°¡ ÀÖ´Â °ÍÀ¸·Î º¼ ¼ö ÀÖ´Ù. ¨é rotiferÀÇ ¹è¾çÁ¤µµ¿¡ µû¶ó CH ±Þ¿©·®ÀÌ ´Þ¶óÁú ¼ö ÀÖ´Ù. Áï, Ãʱâ set-up ´ç½Ã CH ±Þ¿©·®°ú ÀÌÈÄ ±Þ¿©·®ÀÌ ´Þ¶óÁú ¼ö ÀÖ´Ù. ¨ê Á¢Á¾ ÈÄ 3ÀÏ ÈÄ¿¡ recovery ÇÑ´Ù. 3ÀÏ ÀÌÈĺÎÅÍ´Â water quality°¡ ±Þ°ÝÈ÷ ³ªºüÁ®, rotifer ¾çÀÌ ±Þ°ÝÈ÷ °¨¼ÒÇϰųª Á״´Ù. ¨ë CH º¸°ü ¶Ç´Â ±Þ¿© ½Ã, CHÀÇ »ö»óÀÌ green ¡æ brownÀ¸·Î º¯Çß´Ù¸é »ç¿ëÇÏÁö ¸»°í, Æó±âÇÑ´Ù. ¨ì Set-up ½Ã, ÇÏ·ç 2ȸ Á¤µµ´Â rotifer¸¦ 1ml äÃëÇÏ¿© Çö¹Ì°æÀ¸·Î °üÂûÇغ»´Ù. 1. ¿òÁ÷ÀÓ : È°¹ßÈ÷ ¿òÁ÷Àϼö·Ï ÁÁÀº »óÅ 2. ¹è¾ç¾×ÀÌ brown color ÀÏ ¶§, ħÀü¹° È®ÀÎ : ±ú²ýÇÑ brown colorÀÏ ¶§, ħÀü¹°ÀÌ °ÅÀÇ ¾øÀ½. 3. ¾Ë °¹¼ö : ¹è¾ç Á¶°ÇÀÌ ÁÁÀ¸¸é, 3~4°³ Á¤µµÀÇ ¾ËÀ» ´Þ°í ÀÖ´Â rotifer °üÂû °¡´É
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