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 ¿äÁò zebrafish ½ÇÇè¿¡ °ü½ÉÀÌ ¸¹¾Æ¼­ ±Ã±ÝÇÑ Á¡ÀÌ »ý°å´Âµ¥¿ä

 zebrafish ¸¶Ã븦 ½ÃÅ°°í ½Í¾î¼­ ¸¶ÃëÇÏ´Â ¹æ¹ýÀ» ã¾Æº¸°í ÀÖ½À´Ï´Ù.

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¿î¿µÀÚ 12-11-23 21:04
 
ÀϹÝÀûÀ¸·Î ¾²´Â°ÍÀÌ tricaineÀÔ´Ï´Ù.

tricaine(3-amino benzoic acidethylester) Á¦Á¶¹æ¹ý

Stock solution
400mg tricaine powder
97.9ml DD water
~2.1ml 1M Tris(pH9), Adjust pH to ~7

working solution
4.2ml stock solution
~100ml clean tank water

´õ ±Ã±ÝÇϽÅÁ¡ÀÌ ÀÖÀ¸½Ã¸é
jaehoryu@zebrafish.co.kr
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ÇãÀ¯¸® 12-11-23 21:21
 
³× zebrafish book¿¡¼­ tricaine ¸¶Ãë¹ý¿¡ ´ëÇؼ­ Á¦Á¶¹ýÀ» º¸¾Ò´Âµ¥¿ä

ÀÌ°ÍÀ» »ç¿ëÇÒ ¶§, Ä¡¾î¸¦ ¸¶Ã븦 ÇÒ ¼ö ÀÖ´ÂÁö°¡ ±Ã±ÝÇÕ´Ï´Ù.

´Ù Å« ¼º¾î¸¦ ¸¶Ã븦 Çϴ°ÇÁö, ÀÛÀº Ä¡¾îµµ ¸îÀÏ »ý ºÎÅÍ ¸¶Ãë°¡ °¡´ÉÇϸç

¸¶Ã븦 ÇÒ ¶§ ½½¶óÀ̵忡 Ä¡¾î¸¦ ¿Ã·Á³õ°í Çѹæ¿ï¾¿ ¶³¾îÆ®¸®´Â °ÇÁö

¾Æ´Ï¸é ¸¶ÃëÁ¦¿¡ Ä¡¾îµéÀ» ³Ö¾î ¸¶Ã븦 ½ÃÅ°´Â°ÇÁö ³Ê¹« ÀÚ¼¼ÇÏ°í ¹°¾îº¸´Â°Ç°¡¿ä¤Ð¤Ð
¿î¿µÀÚ 12-11-26 18:25
 
larval(Ä¡¾î), adult zebrafish ¸ðµÎ°¡ °¡´ÉÇÕ´Ï´Ù.

larval zebrafish´Â º¸Åë 50~100ea/petridish(90mm)¿¡ egg water¿¡ Å°¿ì½Ã¸é 4ÀϺÎÅÍ ¿òÁ÷À̱â

½ÃÀÛÇÏ´Ï ±×¶§ºÎÅÍ °¡´ÉÇÕ´Ï´Ù. larval´Â ºü¸£°Ô ¸¶Ãë°¡ µÇ°í adult´Â 1minÁ¤µµ ½Ã°£ÀÌ ¼Ò¿äµÇ°í

º¸Åë petridish¿¡¼­ egg water¸¦ »©³»°í tricaine(working soln)À» ³Ö½À´Ï´Ù. Á¤È®ÀÌ ³óµµ°¡ ¸ÂÁö

¾Ê°ÚÁö¸¸ ¸¶Ã븸 ½ÃÅ°¸é µÇ´Ï ¹®Á¦°¡ ¾ø½À´Ï´Ù. ±×¸®°í methylcellulose¿¡ °íÁ¤½ÃÄѼ­ ÀÛ¾÷À» ÇÏ

¸é µË´Ï´Ù. ¾ðÁ¦±îÁö ¸¶Ãë°¡ µÇ´ÂÁö´Â ÃøÁ¤À» ¾ÈÇغÁ¼­(?) ÀϹÝÀûÀÎ »çÁø ÀÛ¾÷Çϴµ¥´Â ¹®Á¦°¡

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